Invariant Natural Killer T‐Cell Receptors Bridge Innate and Adaptive Immunity - pediagenosis
Article Update

Thursday, April 29, 2021

Invariant Natural Killer T‐Cell Receptors Bridge Innate and Adaptive Immunity

Invariant Natural Killer T‐Cell Receptors Bridge Innate and Adaptive Immunity
The highly variable nature of the TCR confers on the conventional T‐cell population the ability to respond to an immense array of different antigens, with individual T‐cells specific for a single antigen. Invariant natural killer T‐cells (iNKT) are a unique subset of T‐cells that display a semi‐variant TCR that equips individual iNKT cells with the ability to detect a broad array of microbial lipid antigens, presented on CD1d antigen‐presenting molecules on antigen‐presenting cells (APCs). Although conventional T‐cells are activated by APCs that have first been activated by microbial antigen (in a process that takes some time), iNKTs can respond directly to PAMPs, secreting cytokines and presenting co‐stimulatory molecules in a manner more reminiscent of innate immune cell PRR activation than T‐cell stimulation.

Natural killer T‐cells

Figure 4.15 Natural killer T‐cells. (a) Schematic representation of type I and type II natural killer T (NKT) cells. These two subsets use different variable (V) region gene segments in the α and β chains of their T‐cell receptors (TCRs), and they recognize different CD1d‐ restricted antigens. (b) The αβTCR is composed of two chains, with the V domains containing the complementarity determining region (CDR) loops. The CDR3 loops are encoded by multiple gene segments and also contain nontemplated (N) regions, which add further diversity to the TCR repertoire. The color coding is the same as that used for the type I NKT TCR in (a). APC, antigen‐presenting cell; C, constant; D, diversity; J, joining. (Reproduced with permis­ sion from the authors Rossjohn et al., (2012) Nature Reviews Immunology 12, 845–857 © Nature Publishing Group.)

Although conventional CD4+ T‐cells provide help to B‐ cells as part of an adaptive immune response, iNKTs are unique in that they can provide help to B‐cells in an innate and adaptive manner, with differing outcomes. iNKTs that are activated by antigen presented on B‐cell C1d can directly license B‐cell activation in a cognate, innate‐like manner, through co‐stimulation with CD40L and the production of various cytokines, such as IFNγ and IL‐21. This leads to a restricted form of B‐ cell activation, with plasmablast expansion, early germinal center development, modest affinity maturation, and primary class‐switched antibody production, but lacking the development of plasma cells and B‐cell memory responses. Alternatively, iNKTs that have been activated by DCs presenting antigen can drive full B‐cell activation in a noncognate, or adaptive fashion, by enlisting the help of CD4+ T‐cells to license B‐cells, driving the generation of mature germinal centers, robust affinity maturation, the development of antibody‐producing plasma cells, and a B‐cell memory response.

Antigen binding by iNKT receptors
Similar to conventional T‐cells, the TCR of iNKTs consists of an α and β chain, with each chain divided into a constant (C) and variable (V) region, with the variable regions conferring ligand diversity. The variable domain of the TCR α chain is split into V and J regions, whereas the β chain is encoded by V, D, and J domains (Figure 4.15). Three complementarity determining regions (CDRs) are situated within the V domains of both α and β chains and these regions enerate the antigen‐binding site of the receptor (Figure 4.15).
There are two main subsets of iNKT cells, type I and type II iNKT, with ligand binding by type I TCRs exhibiting characteristics of innate PRRs, whereas type II iNKT receptors share some similarity with conventional TCRs. Type I cells possess a semi‐invariant TCR α chain (Vα24 Jα18), combined with a restricted β chain which utilizes Vβ11. The CD3α loop plays a particularly important role for type I NKT as deletion of this region in mice greatly impairs ligand binding. A defining characteristic of type I iNKT over type II cells is their ability to detect CD1d‐bound α‐galactosyl­ ceramide (αGalCer), a glycolipid originally isolated from the marine sponge Agelas mauritianus and more recently shown to be a component of Bacteroides bacteria which inhabit the human gut. CD1d binding of αGalCer is typical of other α‐ linked glycolipids, with the hydrophobic portion of the ligand buried in the two main binding regions of CD1d, the A′ and F′ pockets, while the polar region is exposed to solvent.
Binding of ligand by type I TCR is a relatively rigid affair, regardless  of  the  nature  of  the  ligand,  and is  dominated by germline‐encoded regions in the semi‐variant α chain, with assistance from β chain motifs. The type I iNKT TCR positions itself parallel, above the F′ pocket of CD1d, in a manner similar to innate‐like PRRs, with the β chain CDR2β binding a region above the F′ pocket. The αGalCer is bound directly by the CDR1α loop and bridging of both CD1d and αGalCer by the CDR3α loop stabilizes the inter­ action (Figure 4.16). The importance of the CDR2β and CDR3α loops to ligand binding is illustrated by severely reduced ligand binding in receptors bearing mutations to critical residues in these regions. Many microbial ligands have been demonstrated for type I iNKT cells, including α‐ glucosyldiacylglycerols from Streptococcus pneumoniae and α‐galactosyldiacylglycerols from Borrelia burgdorferi. As with αGalCer, the α their microbial origin as mammalian glycolipids have mainly β‐glycosidic linkages.
Type II iNKT cells do not respond to α‐glycolipids such as αGalCer, however, type II receptors possess a more varied, oli­goclonal repertoire than type I cells that share features of both conventional and innate‐like T‐cells. Type II iNKT specific for the self glycolipid antigen sulfatide have been found to play a role in the regulation of various autoimmune disorders, includ­ ing concanavalin A‐induced hepatitis (a mouse model of human autoimmune hepatitis) and type 1 diabetes. The crystal structure of the type II Vα1Jα26–Vβ16Jβ2.1 TCR bound to CD1d/sulfatide shows that, in contrast to the type I receptor, where innate‐like germline‐encoded regions confer specificity, non‐germline‐encoded regions, more similar to conventional TCRs, dominate type II iNKT ligand binding (Figure 4.16). Although the type I receptor is orientated above the CD1d F′ pocket, the type II TCR is positioned above the A′ pocket with both the α and β chains making contact with CD1d in a diagonal orientation, similar to MHC–TCR interactions. Additionally, the CDR3β loop of the type II receptor determines specificity for sulfatide while the α chain of the type I TCR interacts with ligand. The more diverse nature of type II TCR binding is thought to confer on type II iNKT cells the ability to respond to a more varied range of antigens, including sulfatides and other glycolipids, phospholipids, and nonlipid antigens. The ability of type I and II iNKT TCRs to bind CD1d‐presented antigen in mechanistically distinct ways illustrates the impressive nature of mammalian immune systems in aried range of pathogen products.
Structural comparison between type I and type II NKT TCR–lipid–CD1d complexe

Figure 4.16 Structural comparison between type I and type II NKT TCR–lipid–CD1d complexes. (a) The figure shows the docking mode of the T‐cell receptor (TCR) in a type I natural killer T (NKT) cell TCR–lipid–CD1d complex (left) and a type II NKT TCR–lipid–CD1d complex (right). The CD1d antigen‐binding pockets are labelled A′ and F′. (b) The figure shows the view looking down into the antigen‐binding groove of the two complexes showing the parallel docking mode in the type I NKT–lipid–CD1d complex (left), and the orthogonal docking mode in the type II NKT–lipid–CD1d complex (right). Dashed lines represent the docking mode. β2m, β2‐microglobulin. (Reproduced with permission from the authors Rossjohn et al., (2012) Nature Reviews Immunology 12, 845–857 © Nature Publishing Group.)

Share with your friends

Give us your opinion

Note: Only a member of this blog may post a comment.

This is just an example, you can fill it later with your own note.