THE HANDLING OF ANTIGEN - pediagenosis
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Thursday, September 24, 2020

THE HANDLING OF ANTIGEN

THE HANDLING OF ANTIGEN

Where does antigen go when it enters the body? If it penetrates the tissues, it will be carried by the lymph to the draining lymph nodes. Antigens that are encountered in the upper respiratory tract, intestine, or reproductive tract are dealt with by the local MALT, whereas antigens in the blood provoke a reaction in the spleen.


Macrophages are general antigenpresenting cells

Antigens draining into lymphoid tissue are taken up by macrophages. The antigens are then partially, if not completely, broken down in the phagolysosomes; some may escape from the cell in a soluble form to be taken up by other APCs such as dendritic cells, and a fraction may reappear at the surface as a processed peptide associated with MHC class II. Although resting resident macrophages express very little if any MHC class II, antigens are usually encountered in the context of a microbial infectious agent that can activate the macrophage to express class II following engagement of pattern recognition receptors, such as TLR4 by bacterial lipopolysaccharide (LPS). Macrophages are also induced to express MHC class II following exposure to IFNγ or engagement of CD35 (complement receptor 1).

Interdigitating dendritic cells (IDCs)

Figure 6.18 Interdigitating dendritic cells (IDCs). (a) Scanning electron micrograph of a veiled cell, the morphological form adopted by IDCs as they travel in the afferent lymph. (Source: Image courtesy of G.G. MacPherson.) (b) IDC in the thymusdependent area of the rat lymph node. Intimate contacts are made with the surface membranes (arrows) of the surrounding Tlymphocytes (TL) (×2000). In contrast to these IDCs that present processed antigen to Tcells, the follicular dendritic cells in germinal centers present intact antigen to Bcells.

Interdigitating dendritic cells present antigen to naive Tlymphocytes

Notwithstanding the impressive ability of the mighty macrophage to present antigen, there is one function where it is deficient, namely the priming of naive Tlymphocytes. This is the role of the interdigitating dendritic cell (IDC), the crème de la crème of the APCs. Precursors in the blood enter the tissues and differentiate into dendritic cells with phagocytic and endocytic activity. These are sometimes described in the literature as immature dendritic cells but the reality is that they are fully able at this stage to carry out the functions required of them, primarily antigen detection and uptake. These cells include the Langerhans cells in the epidermis of the skin. Receptors involved in antigen capture, including the mannose receptor, various TLRs, and Fc receptors for both IgG and IgE, are present on these dendritic cells. The expression of cell surface MHC class II, and of adhesion and costimulatory molecules, is low at this early stage of the dendritic cells’ life. However, as they differentiate into fully fledged APCs, they decrease their phagocytic and endocytic activity, show reduced levels of molecules involved in antigen capture, but dramatically increase their MHC class II. Costimulatory molecules, such as CD40, CD80 (B7.1), and CD86 (B7.2), are also upregulated. Their expression of a number of chemokine receptors, including CCR7, CCR8, and CXCR4 (see Table 8.2) means that they are attracted to and migrate into Tcell areas in lymphoid tissue.

Two separate developmental pathways for IDCs have been described: the myeloid pathway, which generates CD11c+ interstitial myeloid dendritic cells and skin Langerhans cells, and the lymphoid pathway, which produces plasmacytoid dendritic cells that lack or express only very low levels of CD11 and can produce large amounts of interferon α and β. There appear to be a number of sub­ populations of myeloid dendritic cells, although this area is still somewhat shaky.

In the absence of activation, dendritic cells lack expression of costimulatory molecules such as CD80 and CD86. Antigen presented by these “tolerogenic” dendritic cells will cause Tcell anergy or deletion owing to a lack of costimulation, or will induce regulatory Tcells to secrete immunosuppressive cytokines such as IL10 and TGFβ. Indeed, the dendritic cells themselves are also able to secrete these cytokines. In some circumstances dendritic cells can also exhibit a regulatory phenotype by secreting indoleamine 2,3dioxygenase (IDO), which catalyzes the depletion of trytophan, in the absence of which Tcells undergo apoptosis.

The scenario for Tcell priming appears to be as follows. Dendritic cells pick up and process antigen. As differentiation in response to pattern recognition receptor stimulation proceeds, they downregulate the adhesion molecule Ecadherin, upregulate certain chemokine receptors including CCR7 (which detects CCL19 and CCL21 expressed by the endothelium in peripheral lymph nodes), and produce matrix metalloproteinases to facilitate their migration. They then travel as “veiled” cells in the lymph (Figure 6.18a) before settling down as IDCs in the paracortical Tcell zone of the draining node (Figure 6.18b). There the IDC delivers the processed protein antigen in the form of peptideMHC together with costimulatory signals (Figure 6.19) for potent stimulation of naive, and subsequently of activated, specific Tcells. We will meet IDCs again in Chapter 10 when wediscuss their central role within the thymus in presenting self peptides to developing autoreactive Tcells and triggering their apoptotic execution (known more gently as “clonal deletion”).

 

Migration of interdigitating dendritic cells (IDCs)

Figure 6.19 Migration of interdigitating dendritic cells (IDCs). The precursors of the IDCs are derived from bone marrow stem cells. They travel via the blood to nonlymphoid tissues. At this stage in their life these IDCs (e.g., Langerhans cells in skin) are specialized for antigen uptake. Subsequently they travel via the afferent lymphatics as veiled cells (see Figure 6.18a) to take up residence within secondary lymphoid tissues (see Figure 6.18b) where they express high levels of MHC class II and costimulatory molecules such as B7 (CD80 and CD86). These cells are highly specialized for the activation of naive Tcells. The activated Tcell may carry out its function in the lymph node or, after imprinting with relevant homing molecules, recirculate to the appropriate tissue.

Follicular dendritic cells bind immune complexes and stimulate Bcells

The immunoglobulin receptors FcγRIIB and FcεRII, together with the complement receptors CR1 (CD35) and CR2 (CD21), on the surface of the nonphagocytic MHC class IInegative follicular dendritic cells (FDCs) enables these cells to bind immune complexes of antigen–antibody–complement very efficiently. Memory Bcells can then be stimulated by recognition of the antigen, and costimulated through the Bcell CD21 recognizing complement fragments on the surface of the FDC. Intact antigens can be retained as immune complexes on FDCs for many months or possibly even longer.


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